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Gluten Free Brewing With Enzymes

I am following Thirstyboys threads on experimental brewing with gluten free grains and then enzymes, and then I am willing to have a go.baking enzymes flour
Gluten Free Brewing With Enzymes
I have got gotten my mitts on some enzymes, pullulanase, beta-glucanase, alpha-amylase, protease, and then gluco-amylase(amyloglucosidase).
However, I don't have a lot associated with these as they are trial packs bagsdelivered originating from a company in Auckland, well, i should make i have the process just before I start.
I am targeting 5L batches to begin with, so my recipe together with protocol is really as follows, modified from Andrew (Millet man)baking enzymes buy
1/2 kg millet grain
1/2 kg sorghum syrup
Crush grain and mix with 1.0 lt water not to mention add pullulanase, beta-glucanase, alpha-amylase, coupled with protease.
30 min at 55C
30 min at 85C
Add cold water to reduce temperature and also add gluco amylase
120 ...
... min at 65C
Then lauter and sparge, and additionally continue as normal adding the 1/2kg relating to sorghum syrup for the kettle.enzymes baking bread
My concern is determining the amount of associated with each enzyme to add. We have calculated on the maker plus online that 1KNU(T) is the quantity of alpha amylase which at pH7.1 37C dextrinises 5.26g starch dry substance every hour. Thus i will require 115 KNU(T)'s per kilo associated with grain. ~1g connected with my alpha amylase per kilo related with grain. Will the work better or faster at a higher temp not to mention lower pH?
But what about the others? Is there a way related with calculating this or do you just play around not to mention see what are the results?
Is it ok to brew a 5L batch in a standard 30L fermenter or perhaps there too much headspace?enzymes baking food
Any ideas, tips, or tricks less complicated appreciated.c
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