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Benefits Of Using Hplc Instruments
High Performance Liquid Chromatography or better known as HPLC is a technique for analyzing complex chemical compounds. Recent years have witnessed a huge increase in demand for various HPLC instruments by the scientific community. HPLC instruments are widely used by pharmaceutical companies, petro chemical companies, food & drug institutes and many more. Today, there are various brands of HPLC instruments available in the market which you can choose depending on your scientific requirements.
One of the major areas where HPLC is used is drug analysis. These advanced research instruments use large amounts of pressure to force the chemical compound through the column. Now this is ideal for larger molecules than GLC/GSC, but still provides a reasonable amount of detail about the identity of the molecules. HPLC instruments have very small holes for the compound to pass through, giving better separation of similar compounds.
Another important benefit offered by HPLC is that it helps researchers quickly separate compounds on the basis of their polarity. Accurate results can be accomplished as per different ...
... research requirements. With the help of a pressurized pump, an HPLC instrument helps separate compounds with great precision. One can get results within minutes. These equipments are considered to be a boon for researchers involved in drug discovery and bio analytical studies.
Most of the scientists, today, prefer HPLC over Gas Chromatography. Why? Well, there are many reasons which have helped HPLC gain an edge over GC. To begin with, in gas chromatography, samples injected into the system are vaporized at approx 400 degrees Fahrenheit before these can pass through the column. What happens here is that, a compound might not be able to withstand such head and gets degraded into another molecule. On the other hand, LC helps researchers in analyzing larger and less stable compounds because these are not subjected to heat.
HPLC autosamplers help researchers in injecting samples into continuously flowing mobile phase stream which carries the sample into the column. This HPLC part contains the chromatographic packing material needed to effect the separation. This packing material is called the stationary phase because it is held in place by the column hardware.
With the help of HPLC detectors, one can quickly separate compounds. Here ultraviolet rays are thrown at sample components as they come out of column. Usually organic compounds absorb light, now as they pass through the applied light beam; a detector can help know how much of that light was absorbed. Further, this HPLC part also records the retention time of the components based on the order in which they come off the column. With this, researchers can quickly analyze the exact nature of the sample's components.
As a scientist, you must always select an experienced online supplier of HPLC instruments. The HPLC parts offered by them are best in quality and come with appropriate warranty too.
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