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Cdna Library Construction - An Effective Molecular Appraoch For Indepth Analysis
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An overview of cDNA Library Construction
cDNA is obtained from transcribed mRNA which is extracted from the nucleus of the cell and consists of expressed genes of a living organism. cDNA libraries are a powerful source for identifying gene products. Created from a mature mRNA that is obtained from the eukaryotic cell using reverse transcriptase, it can be effectively used as a primer site for reverse transcription.
With ever evolving technology and fast advancing medicine, we have come to a point where every fatal disease can be diagnosed and treated using genomic research. Bioinformaticians and scientists are working day and night in researching new tools and techniques to cure diseases like cancer and HIV. They are inventing new vaccines and drugs that are effective in curing difficult to treat diseases.
Other uses of cDNA Library Construction
Use in the reproduction of eukaryotic genomes
To express eukaryotic genes in prokaryotes
For reverse genetics when the supplementary genomic information is rendered useless
All the clones at CD Genomics are accurately oriented and precisely screened for antibodies and expressions in a custom vector, unlike other random procedures used for library construction.
Genomic research has played a vital role in the invention of chemotherapy and radiotherapy which have proven efficacy in curing cancer. This has considerably brought down the death ratio and increased the life expectancy rate across the globe.
With the invention and development of cDNA library, bioinformaticians and researchers are now able to judge reunion kinetics which have the capacity to reprimand the high inconsistency in profusion among the cDNA clones which depict character mRNA species.
cDNA libraries constructed from microdissected tissues can generate gene expression profiles of tumors of distinct histology and tumor stages. The quality of a cDNA library can be assessed using a combination of the average length of the inserts, the percentage of short clones and the relative amount of contaminating background clones.
cDNA Library Construction Service at CD Genomics
Our highly advanced technology and latest platforms allow investigation of the suspected mRNA pool to disclose differentially expressed mRNAs and also facilitate the study of orientation of spatial forms in any organism. Our scientists have constructed several libraries using an array of resources including humans, animals, plants, microbes, fungi, yeast, protoplast and other bacterial cells.
Our vast domain of services integrates pioneering techniques so that you get high quality and reliable results. Our cDNA libraries contain DNA copies for all transcripts which are characteristic of that particular biological article which is taken under the scope of the study.
Normalized cDNA libraries are basically used for the discovery of novel genes that are expressed in the investigated biological objects. These library construction services are apt for EST sequencing as well as gene discovery applications. CD Genomics also sequences ESTs from cDNA libraries via Sanger sequencing.
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